Hotspot

ndhD

SSC · 119883-121244 · 1362 bp

Back to Boswellia

Pi 0.00189

MarkerSeek score 34.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1362
variable_sites: 9
pi: 0.00189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAAGGCGTCTGGTTCCAATT	AAACAACTCGAAGCATGGGT	45.0	45.0	58.0	58.0	1854-1890 bp	1.0	82.6
ndhD_p2	AAAGGCGTCTGGTTCCAATT	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.0	58.0	1948-1984 bp	1.0	82.5
ndhD_p3	AAAGGCGTCTGGTTCCAATT	GGCATGAAACAACTCGAAGC	45.0	50.0	58.0	58.0	1860-1896 bp	1.0	82.5
ndhD_p4	AAAGGCGTCTGGTTCCAATT	CTGCTCCAAGAACAGAGGAC	45.0	55.0	58.0	57.9	1947-1983 bp	1.0	81.9
ndhD_p5	AAAGGCGTCTGGTTCCAATT	TGCTTCTGCTCCAAGAACAG	45.0	50.0	58.0	58.1	1952-1988 bp	1.0	81.7