Hotspot

ccsA-ndhD

SSC · 119550-119882 · 333 bp

Back to Boswellia

Pi 0.0389

MarkerSeek score 76.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 333
variable_sites: 42
pi: 0.0389
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTGGCTTCTATGGGCTTTC	CGTGGACAAAGTCGAAGCTA	50.0	50.0	57.9	57.9	444-476 bp	1.0	89.7
ccsA-ndhD_p2	TGTGGCTTCTATGGGCTTTC	ATCCGTGGACAAAGTCGAAG	50.0	50.0	57.9	57.9	447-479 bp	1.0	89.5
ccsA-ndhD_p3	TTCCGCAATTGTGGCTTCTA	CGTGGACAAAGTCGAAGCTA	45.0	50.0	57.8	57.9	453-485 bp	1.0	89.0
ccsA-ndhD_p4	TTCCGCAATTGTGGCTTCTA	ATCCGTGGACAAAGTCGAAG	45.0	50.0	57.8	57.9	456-488 bp	1.0	88.8
ccsA-ndhD_p5	AATTCCGCAATTGTGGCTTC	CGTGGACAAAGTCGAAGCTA	45.0	50.0	57.6	57.9	455-487 bp	1.0	87.6