Hotspot

rpl32-trnL-UAG

SSC · 117155-118388 · 1234 bp

Back to Boswellia

Pi 0.0167

MarkerSeek score 63.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1234
variable_sites: 63
pi: 0.0167
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCGTTGAAAGCTTTTTCGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1232-1364 bp	1.0	86.5
rpl32-trnL-UAG_p2	GGCGTTGAAAGCTTTTTCGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1233-1365 bp	1.0	86.5
rpl32-trnL-UAG_p3	GGCGTTGAAAGCTTTTTCGT	TCGAACCGAGATGCTTTAGC	45.0	50.0	58.2	58.1	1268-1400 bp	1.0	85.8
rpl32-trnL-UAG_p4	GGCGTTGAAAGCTTTTTCGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	1242-1374 bp	1.0	85.5
rpl32-trnL-UAG_p5	GGCGTTGAAAGCTTTTTCGT	TCCAATCGAAACGACCGAAA	45.0	45.0	58.2	57.8	1457-1583 bp	1.0	85.2