Hotspot

rpoB-trnC-GCA

LSC · 28152-29385 · 1234 bp

Back to Boswellia

Pi 0.00394

MarkerSeek score 57.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1234
variable_sites: 16
pi: 0.00394
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GCATTTCCATCTCCGAGCAT	TTGTTAATCAGGCGACACCC	50.0	50.0	58.4	58.2	1329-1342 bp	1.0	77.1
rpoB-trnC-GCA_p2	GCATTTCCATCTCCGAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1293-1306 bp	1.0	75.4
rpoB-trnC-GCA_p3	TTTCCGCATTTCCATCTCCG	TTGTTAATCAGGCGACACCC	50.0	50.0	58.6	58.2	1334-1347 bp	1.0	75.3
rpoB-trnC-GCA_p4	GCATTTCCATCTCCGAGCAT	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1318-1331 bp	1.0	74.5
rpoB-trnC-GCA_p5	GCATTTCCATCTCCGAGCAT	TAATCAGGCGACACCCAGAT	50.0	50.0	58.4	58.5	1325-1338 bp	1.0	74.3