Hotspot

ycf80

Genome · 53229-54683 · 1455 bp

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Pi 0.243

MarkerSeek score 81.70

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1455
variable_sites: 344
pi: 0.243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	AAGAGCATGTAACTCAGCGG	TACCAGCTGGAGAACATGGA	50.0	50.0	58.0	58.0	2673 bp	0.5	59.5
ycf80_p2	TCCCGCCAGTTCCATTTATG	TACCAGCTGGAGAACATGGA	50.0	50.0	57.9	58.0	2104 bp	0.5	59.2
ycf80_p3	AATTCCCGCCAGTTCCATTT	TACCAGCTGGAGAACATGGA	45.0	50.0	58.1	58.0	2107 bp	0.5	59.1
ycf80_p4	AAGAGCATGTAACTCAGCGG	CAGCTGGAGAACATGGACAA	50.0	50.0	58.0	57.8	2670 bp	0.5	58.4
ycf80_p5	ATGGACGTGGGTTCAATTCC	TACCAGCTGGAGAACATGGA	50.0	50.0	58.2	58.0	2121 bp	0.5	58.3