Hotspot

syfB

Genome · 155740-157719 · 1980 bp

Back to Bostrychia

Pi 0.237

MarkerSeek score 80.00

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 1980
variable_sites: 458
pi: 0.237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	TGCCACAGAAATATACCGCC	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.0	58.0	2773-2800 bp	1.0	89.5
syfB_p2	AAACCCGGCTTACGTCTTAC	CGAATCCTCCTACCCCAGAT	50.0	55.0	57.9	58.0	2493-2511 bp	1.0	88.9
syfB_p3	TGCCACAGAAATATACCGCC	GGTGTAGCCAAGTGGTAAGG	50.0	55.0	58.0	57.9	2824-2851 bp	1.0	88.7
syfB_p4	TACCGCCTGTTGTTTGTTCA	CGAATCCTCCTACCCCAGAT	45.0	55.0	58.0	58.0	2760 bp	0.5	59.5
syfB_p5	AGGAAAGTCCGGGCTCTATT	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.1	58.0	2842 bp	0.5	58.9