Hotspot

rps3

Genome · 121388-122032 · 645 bp

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Pi 0.157

MarkerSeek score 79.70

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 645
variable_sites: 101
pi: 0.157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TTGCTGACAAAGGTCCAACA	AACCCATTCTTGTTTCGGCT	45.0	45.0	57.9	58.0	1059 bp	0.5	58.9
rps3_p2	GCTTTTGCTGACAAAGGTCC	AACCCATTCTTGTTTCGGCT	50.0	45.0	57.9	58.0	1063 bp	0.5	58.8
rps3_p3	TTGCTGACAAAGGTCCAACA	CATTTCCTGTCATACGGCCA	45.0	50.0	57.9	58.0	867 bp	0.5	58.6
rps3_p4	TTGCTGACAAAGGTCCAACA	ATTTCCTGTCATACGGCCAC	45.0	50.0	57.9	58.0	866 bp	0.5	58.6
rps3_p5	GCTTTTGCTGACAAAGGTCC	CATTTCCTGTCATACGGCCA	50.0	50.0	57.9	58.0	871 bp	0.5	58.6