Hotspot

rps1

Genome · 154492-155283 · 792 bp

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Pi 0.201

MarkerSeek score 80.00

Primer available yes

Label rps1

Feature Metadata

label_name: rps1
feature_type: gene
region: Genome
length_bp: 792
variable_sites: 157
pi: 0.201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps1_p1	TCCATTGCCGTAACCTCTTG	GTAAGACGTAAGCCGGGTTT	50.0	50.0	57.9	57.9	1567-1579 bp	1.0	87.7
rps1_p2	TCCATTGCCGTAACCTCTTG	GGCGGTATATTTCTGTGGCA	50.0	50.0	57.9	58.0	1287-1290 bp	1.0	87.5
rps1_p3	ACTCGAACCCGCGTATAATG	GGCGGTATATTTCTGTGGCA	50.0	50.0	57.9	58.0	1316-1319 bp	1.0	87.3
rps1_p4	TCCATTGCCGTAACCTCTTG	TGAACAAACAACAGGCGGTA	50.0	45.0	57.9	58.0	1300 bp	0.5	58.8
rps1_p5	ACTCGAACCCGCGTATAATG	TGAACAAACAACAGGCGGTA	50.0	45.0	57.9	58.0	1329 bp	0.5	58.6