Hotspot

rpl9

Genome · 147886-148350 · 465 bp

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Pi 0.256

MarkerSeek score 75.90

Primer available yes

Label rpl9

Feature Metadata

label_name: rpl9
feature_type: gene
region: Genome
length_bp: 465
variable_sites: 119
pi: 0.256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl9_p1	AATCGCCATTTGGTAGTCCC	TGGAGCAGTAGAGGGATTTGA	50.0	47.6	57.9	58.2	1845 bp	0.5	50.2
rpl9_p2	AACAATTTTGTCAAGGCCGC	TGGAGCAGTAGAGGGATTTGA	45.0	47.6	58.1	58.2	1466 bp	0.5	49.6
rpl9_p3	AATCGCCATTTGGTAGTCCC	TGGAGCAGTAGAGGGATTTG	50.0	50.0	57.9	56.6	1845 bp	0.5	48.5
rpl9_p4	TCCCATAGATGGTCTACCGG	TGGAGCAGTAGAGGGATTTGA	55.0	47.6	57.7	58.2	1877 bp	0.5	48.2
rpl9_p5	AACAATTTTGTCAAGGCCGC	TGGAGCAGTAGAGGGATTTG	45.0	50.0	58.1	56.6	1466 bp	0.5	47.9