Hotspot

infB

Genome · 87648-89864 · 2217 bp

Back to Bostrychia

Pi 0.227

MarkerSeek score 78.60

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2217
variable_sites: 497
pi: 0.227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	TTGTCTCTGTTGCCATTTTT	TGTTCCCAGTATTGAGCAGC	35.0	50.0	53.9	57.9	2918 bp	0.5	26.1
infB_p2	TTTGTCTCTGTTGCCATTTT	TGTTCCCAGTATTGAGCAGC	35.0	50.0	53.9	57.9	2919 bp	0.5	26.1
infB_p3	TTTTGTCTCTGTTGCCATTT	TGTTCCCAGTATTGAGCAGC	35.0	50.0	53.9	57.9	2920 bp	0.5	26.1
infB_p4	TTTTTGTCTCTGTTGCCATT	TGTTCCCAGTATTGAGCAGC	35.0	50.0	53.9	57.9	2921 bp	0.5	26.1
infB_p5	TGTCTCTGTTGCCATTTTTAAACA	TGTTCCCAGTATTGAGCAGC	33.3	50.0	57.8	57.9	2917 bp	0.5	25.4