Hotspot

dsbD

Genome · 22598-23350 · 753 bp

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Pi 0.276

MarkerSeek score 60.60

Primer available yes

Label dsbD

Feature Metadata

label_name: dsbD
feature_type: gene
region: Genome
length_bp: 753
variable_sites: 27
pi: 0.276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.13
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dsbD_p1	TTTCACCACGTCCGCTATTT	CAAATTTCCATCGACGAGCA	45.0	45.0	57.8	56.8	1430 bp	0.5	49.2
dsbD_p2	TTTCACCACGTCCGCTATTT	ACAAATTTCCATCGACGAGC	45.0	45.0	57.8	56.8	1431 bp	0.5	49.1
dsbD_p3	TAGCGTGTCTGCCAATTTCA	CAAATTTCCATCGACGAGCA	45.0	45.0	57.8	56.8	1445 bp	0.5	49.1
dsbD_p4	TAGCGTGTCTGCCAATTTCA	ACAAATTTCCATCGACGAGC	45.0	45.0	57.8	56.8	1446 bp	0.5	49.1
dsbD_p5	CTCAGTGGTAGAGCGATTGG	CAAATTTCCATCGACGAGCA	55.0	45.0	57.8	56.8	1265 bp	0.5	48.8