Hotspot

accB

Genome · 109472-110011 · 540 bp

Back to Bostrychia

Pi 0.243

MarkerSeek score 77.70

Primer available yes

Label accB

Feature Metadata

label_name: accB
feature_type: gene
region: Genome
length_bp: 540
variable_sites: 131
pi: 0.243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accB_p1	ATAGCTCTGCCTACACGACA	AATGACCTGGTTTTGCCCAT	50.0	45.0	58.2	58.0	1556 bp	0.5	57.9
accB_p2	ATAGCTCTGCCTACACGACA	ATGACCTGGTTTTGCCCATT	50.0	45.0	58.2	58.0	1555 bp	0.5	57.9
accB_p3	ATAGCTCTGCCTACACGACA	GTCGTGAGCATCTGCATGTA	50.0	50.0	58.2	58.1	1627 bp	0.5	57.5
accB_p4	ATAGCTCTGCCTACACGACA	AAAGTCGTGAGCATCTGCAT	50.0	45.0	58.2	57.9	1630 bp	0.5	57.3
accB_p5	ATAGCTCTGCCTACACGACA	ATGCCTTGAAATCCTCCACC	50.0	50.0	58.2	57.9	1866 bp	0.5	56.9