Hotspot

ConsOrf4

Genome · 68271-69050 · 780 bp

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Pi 0.298

MarkerSeek score 81.40

Primer available yes

Label ConsOrf4

Feature Metadata

label_name: ConsOrf4
feature_type: gene
region: Genome
length_bp: 780
variable_sites: 226
pi: 0.298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf4_p1	GCAGCCTTGCGAAAATTTCT	TTGCTGAATTTAATGCCGCA	45.0	40.0	57.9	57.0	1789 bp	0.5	51.2
ConsOrf4_p2	AGGAACTGCATACTGAGGGA	TTGCTGAATTTAATGCCGCA	50.0	40.0	57.7	57.0	2150 bp	0.5	49.8
ConsOrf4_p3	TAGTTCAGCACCCCACTCTT	TTGCTGAATTTAATGCCGCA	50.0	40.0	58.3	57.0	1817 bp	0.5	49.6
ConsOrf4_p4	TTAGTTCAGCACCCCACTCT	TTGCTGAATTTAATGCCGCA	50.0	40.0	58.3	57.0	1818 bp	0.5	49.6
ConsOrf4_p5	GCAGCCTTGCGAAAATTTCT	TGCTGAATTTAATGCCGCAT	45.0	40.0	57.9	56.7	1788 bp	0.5	49.4