Hotspot

ConsOrf3

Genome · 161458-161964 · 507 bp

Back to Bostrychia

Pi 0.246

MarkerSeek score 79.70

Primer available yes

Label ConsOrf3

Feature Metadata

label_name: ConsOrf3
feature_type: gene
region: Genome
length_bp: 507
variable_sites: 121
pi: 0.246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf3_p1	TGCTACTAAGGCTGCGATTG	GCCAAGAAATGATGCATGGG	50.0	50.0	58.1	57.8	1142 bp	0.5	57.6
ConsOrf3_p2	TGCTACTAAGGCTGCGATTG	GGCCAAGAAATGATGCATGG	50.0	50.0	58.1	57.8	1143 bp	0.5	57.6
ConsOrf3_p3	TCCTGGTGGTGGTTCTACTT	GCCAAGAAATGATGCATGGG	50.0	50.0	57.9	57.8	1109 bp	0.5	57.2
ConsOrf3_p4	TCCTGGTGGTGGTTCTACTT	GGCCAAGAAATGATGCATGG	50.0	50.0	57.9	57.8	1110 bp	0.5	57.2
ConsOrf3_p5	TTCCTGGTGGTGGTTCTACT	GCCAAGAAATGATGCATGGG	50.0	50.0	57.9	57.8	1110 bp	0.5	57.2