Hotspot

ycf1

SSC · 124830-130319 · 5490 bp

Back to Boronia

Pi 0.0373

MarkerSeek score 61.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5490
variable_sites: 203
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTCGTATTTTCCCAGCTC	CAGGAACAAGAGGGATCTGC	50.0	55.0	57.7	58.0	1097 bp	0.5	57.4
ycf1_p2	GTCGTATTTTCCCAGCTCCC	CAGGAACAAGAGGGATCTGC	55.0	55.0	58.3	58.0	1095 bp	0.5	57.0
ycf1_p3	GCTCCCGGGGAAAATTATCA	CAGGAACAAGAGGGATCTGC	50.0	55.0	57.6	58.0	1081 bp	0.5	56.8
ycf1_p4	CAGCTCCCGGGGAAAATTAT	CAGGAACAAGAGGGATCTGC	50.0	55.0	57.6	58.0	1083 bp	0.5	56.8
ycf1_p5	TCGTCGTATTTTCCCAGCTC	GGGATCTGCCGAAGAAGATC	50.0	55.0	57.7	57.8	1086 bp	0.5	56.4