Hotspot

rps8-rpl14

LSC · 80824-80974 · 151 bp

Back to Boronia

Pi 0.0728

MarkerSeek score 76.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 11
pi: 0.0728
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCAATAGTGTCCTTGCC	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.9	58.0	260-272 bp	1.0	82.2
rps8-rpl14_p2	TCAGCAATAGTGTCCTTGCC	GGGGAACTCGAGTTTTTGGT	50.0	50.0	57.9	58.0	263-275 bp	1.0	82.2
rps8-rpl14_p3	TCAGCAATAGTGTCCTTGCC	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	57.9	58.1	258-270 bp	1.0	81.5
rps8-rpl14_p4	TTCGAACCGTTCCTTTTCGA	GAACTCGAGTTTTTGGTGCG	45.0	50.0	57.8	58.0	313-325 bp	1.0	80.8
rps8-rpl14_p5	TTCGAACCGTTCCTTTTCGA	GGGGAACTCGAGTTTTTGGT	45.0	50.0	57.8	58.0	316-328 bp	1.0	80.8