Hotspot

psbE-petL

LSC · 64737-66128 · 1392 bp

Back to Boronia

Pi 0.0452

MarkerSeek score 66.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1392
variable_sites: 41
pi: 0.0452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCAAAATAACTAGTTATAGTAGGCA	50.0	32.0	57.8	54.4	983-1445 bp	1.0	46.7
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	CCAAAATAACTAGTTATAGTAGGCA	55.0	32.0	58.2	54.4	978-1440 bp	1.0	46.7
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	CCAAAATAACTAGTTATAGTAGGCA	50.0	32.0	58.4	54.4	976-1438 bp	1.0	46.7
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	CCAAAATAACTAGTTATAGTAGGCA	50.0	32.0	58.6	54.4	977-1439 bp	1.0	46.7
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	CCAAAATAACTAGTTATAGTAGGCA	45.0	32.0	57.4	54.4	984-1446 bp	1.0	46.7