Hotspot

psbZ-trnG-GCC

LSC · 36642-37165 · 524 bp

Back to Boronia

Pi 0.0686

MarkerSeek score 81.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 35
pi: 0.0686
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGTCTGGTACATCAGTGTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.9	57.8	639-807 bp	1.0	75.0
psbZ-trnG-GCC_p2	AGTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.8	57.8	611-779 bp	1.0	74.6
psbZ-trnG-GCC_p3	TGGTACATCAGTGTGGATTGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.7	57.8	635-803 bp	1.0	73.3
psbZ-trnG-GCC_p4	TGTCTGGTACATCAGTGTGG	ATATTCCTTTTCCCTGGCGG	50.0	50.0	56.9	57.6	678-846 bp	1.0	73.2
psbZ-trnG-GCC_p5	TGTCTGGTACATCAGTGTGGA	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.4	57.8	639-807 bp	1.0	72.9