Hotspot

atpH-atpI

LSC · 13517-14756 · 1240 bp

Back to Boronia

Pi 0.0394

MarkerSeek score 75.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1240
variable_sites: 48
pi: 0.0394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.0	58.0	1345-1351 bp	1.0	83.6
atpH-atpI_p2	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	1383-1389 bp	1.0	82.8
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1321-1327 bp	1.0	82.8
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.2	58.0	1346-1352 bp	1.0	82.4
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1359-1365 bp	1.0	82.0