Hotspot

trnS-GCU-trnG-UCC

LSC · 7674-8532 · 859 bp

Back to Boronia

Pi 0.0564

MarkerSeek score 73.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 859
variable_sites: 37
pi: 0.0564
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	707-910 bp	1.0	55.5
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	708-911 bp	1.0	54.4
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	765-968 bp	1.0	54.1
trnS-GCU-trnG-UCC_p4	ACGCTTTAATCCACTCAGCC	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	704-907 bp	1.0	53.5
trnS-GCU-trnG-UCC_p5	CGCTTTAATCCACTCAGCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	703-906 bp	1.0	53.5