Hotspot

ycf1

SSC · 128538-134102 · 5565 bp

Back to Borassus

Pi 0.00144

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5565
variable_sites: 8
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATTGTATTTTGATCTGTTTCATCA	CGGAATCACAGAAACATATTTTGC	26.9	37.5	56.0	57.4	246 bp	1.0	40.7
ycf1_p2	AGTTCATTGTATTTTGATCTGTTTCA	CGGAATCACAGAAACATATTTTGC	26.9	37.5	56.1	57.4	249 bp	1.0	40.6
ycf1_p3	GTTCATTGTATTTTGATCTGTTTCA	CGGAATCACAGAAACATATTTTGC	28.0	37.5	54.9	57.4	248 bp	1.0	40.6
ycf1_p4	AGTTCATTGTATTTTGATCTGTTTCA	AAATCGGAATCACAGAAACA	26.9	35.0	56.1	53.1	253 bp	1.0	40.6
ycf1_p5	AAGTTCATTGTATTTTGATCTGTTTCA	CGGAATCACAGAAACATATTTTGC	25.9	37.5	56.7	57.4	250 bp	1.0	40.6