Hotspot

rps3

LSC · 86292-86957 · 666 bp

Back to Borassus

Pi 0
MarkerSeek score 61.50
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
666
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 TGGCAAGACAAATGAAGGTCT ACCCACTTGTCATATAACAATTGT 42.9 33.3 57.8 56.6 798-818 bp 1.0 42.0
rps3_p2 TGGCAAGACAAATGAAGGTCT AACCCACTTGTCATATAACAATTGT 42.9 32.0 57.8 57.2 799-819 bp 1.0 42.0
rps3_p3 TGGCAAGACAAATGAAGGTC ACCCACTTGTCATATAACAATTGT 45.0 33.3 56.2 56.6 798-818 bp 1.0 42.0
rps3_p4 TGGCAAGACAAATGAAGGTCT ACCCACTTGTCATATAACAATTG 42.9 34.8 57.8 54.9 798-818 bp 1.0 42.0
rps3_p5 TGGCAAGACAAATGAAGGTCT CCCACTTGTCATATAACAATTGT 42.9 34.8 57.8 54.9 797-817 bp 1.0 42.0