Hotspot

rps2

LSC · 16500-17210 · 711 bp

Back to Borassus

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	CCGATCCAATAAATCTATGAGTTGC	GTATCAACGGTCAATCCCCC	40.0	55.0	58.2	58.0	872 bp	1.0	40.1
rps2_p2	CGATCCAATAAATCTATGAGTTGCC	GTATCAACGGTCAATCCCCC	40.0	55.0	58.2	58.0	871 bp	1.0	40.1
rps2_p3	CCGATCCAATAAATCTATGAGTTGC	GGTCGTGTATCAACGGTCAA	40.0	50.0	58.2	57.9	878 bp	1.0	40.1
rps2_p4	CGATCCAATAAATCTATGAGTTGCC	GGTCGTGTATCAACGGTCAA	40.0	50.0	58.2	57.9	877 bp	1.0	40.1
rps2_p5	TCCAATAAATCTATGAGTTGCCA	GTATCAACGGTCAATCCCCC	34.8	55.0	55.7	58.0	868 bp	1.0	40.1