Hotspot

rps16

LSC · 4849-5965 · 1117 bp

Back to Borassus

Pi 0.000899

MarkerSeek score 59.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1117
variable_sites: 1
pi: 0.000899
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCAAGAAAAAGGCAAATTGTGT	CGGTTGAGCCAACGACTATT	36.4	50.0	57.1	58.3	1280-1285 bp	1.0	55.1
rps16_p2	GCAAGAAAAAGGCAAATTGTGT	TATACACACACCGGTTGAGC	36.4	50.0	57.1	57.6	1291-1296 bp	1.0	54.3
rps16_p3	ACAGCAAGAAAAAGGCAAATTG	CGGTTGAGCCAACGACTATT	36.4	50.0	56.9	58.3	1283-1288 bp	1.0	53.0
rps16_p4	AGCAAGAAAAAGGCAAATTGTG	CGGTTGAGCCAACGACTATT	36.4	50.0	56.9	58.3	1281-1286 bp	1.0	53.0
rps16_p5	CAGCAAGAAAAAGGCAAATTGT	CGGTTGAGCCAACGACTATT	36.4	50.0	56.9	58.3	1282-1287 bp	1.0	53.0