Hotspot

ccsA

SSC · 118593-119561 · 969 bp

Back to Borassus

Pi 0.00103

MarkerSeek score 49.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTGGCGGCATCCTATAAA	ACTCGAACAAGCAAACTTTCG	50.0	42.9	57.5	57.7	1116-1121 bp	1.0	65.2
ccsA_p2	GAGTGGCGGCATCCTATAAA	CTCGAACAAGCAAACTTTCGT	50.0	42.9	57.5	57.7	1115-1120 bp	1.0	65.2
ccsA_p3	GAGTGGCGGCATCCTATAAA	TCGAACAAGCAAACTTTCGT	50.0	40.0	57.5	56.5	1114-1119 bp	1.0	63.9
ccsA_p4	AGTGGCGGCATCCTATAAAA	ACTCGAACAAGCAAACTTTCG	45.0	42.9	57.0	57.7	1115-1120 bp	1.0	61.3
ccsA_p5	AGTGGCGGCATCCTATAAAA	CTCGAACAAGCAAACTTTCGT	45.0	42.9	57.0	57.7	1114-1119 bp	1.0	61.3