Hotspot

rps15-ycf1

SSC · 128121-128537 · 417 bp

Back to Borassus

Pi 0.00719

MarkerSeek score 59.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 417
variable_sites: 3
pi: 0.00719
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGAACCCTTGTTTTCT	TGGCCCAATTACCGATTAGA	38.1	45.0	55.5	56.3	605 bp	1.0	40.4
rps15-ycf1_p2	TCAACAGAACCCTTGTTTTCTTC	TGGCCCAATTACCGATTAGA	39.1	45.0	57.4	56.3	605 bp	1.0	40.4
rps15-ycf1_p3	CAACAGAACCCTTGTTTTCTTCT	TGGCCCAATTACCGATTAGA	39.1	45.0	57.4	56.3	604 bp	1.0	40.4
rps15-ycf1_p4	TGAAATTCAACAGAACCCTTGT	TGGCCCAATTACCGATTAGA	36.4	45.0	56.4	56.3	611 bp	1.0	40.4
rps15-ycf1_p5	TCAACAGAACCCTTGTTTTCT	TGGCCCAATTACCGATTAGAA	38.1	42.9	55.5	57.1	605 bp	1.0	40.4