Hotspot

ccsA-ndhD

SSC · 119562-119771 · 210 bp

Back to Borassus

Pi 0.019
MarkerSeek score 63.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
210
variable_sites
4
pi
0.019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ACGAATTCGGCACTTATAGCT TCAGTTGACAGGGTACAAGC 42.9 50.0 57.5 57.5 378-383 bp 1.0 64.9
ccsA-ndhD_p2 ACGAATTCGGCACTTATAGCT CAGTTGACAGGGTACAAGCT 42.9 50.0 57.5 57.4 377-382 bp 1.0 64.8
ccsA-ndhD_p3 ACGAATTCGGCACTTATAGC TCAGTTGACAGGGTACAAGC 45.0 50.0 56.0 57.5 378-383 bp 1.0 60.8
ccsA-ndhD_p4 ACGAATTCGGCACTTATAGCT ATCAGTTGACAGGGTACAAGC 42.9 47.6 57.5 57.9 379-384 bp 1.0 60.7
ccsA-ndhD_p5 ACGAATTCGGCACTTATAGC CAGTTGACAGGGTACAAGCT 45.0 50.0 56.0 57.4 377-382 bp 1.0 60.7