Hotspot

petG-trnW-CCA

LSC · 69208-69326 · 119 bp

Back to Borassus

Pi 0.0168

MarkerSeek score 62.20

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 2
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	223 bp	1.0	79.4
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	187 bp	1.0	78.9
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	AGGTTCAAATCCTGCAGAGC	50.0	50.0	57.8	58.2	171 bp	1.0	78.3
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	221 bp	1.0	76.7
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	CCCGATGTCGTAGGTTCAAA	50.0	50.0	57.8	57.6	182 bp	1.0	76.7