Hotspot

trnF-GAA-ndhJ

LSC · 49761-50488 · 728 bp

Back to Borassus

Pi 0.00138

MarkerSeek score 60.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 1
pi: 0.00138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	834-838 bp	1.0	79.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	824-828 bp	1.0	78.5
trnF-GAA-ndhJ_p3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	55.0	50.0	57.8	57.9	880-884 bp	1.0	78.1
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	877-881 bp	1.0	77.9
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	876-880 bp	1.0	77.9