Hotspot

trnS-GCU-trnG-UCC

LSC · 8420-9797 · 1378 bp

Back to Borassus

Pi 0.00605

MarkerSeek score 66.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1378
variable_sites: 8
pi: 0.00605
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AACTGAGAGACCCCTTAACT	45.0	45.0	58.0	55.1	1490-1564 bp	1.0	59.6
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AAACTGAGAGACCCCTTAACT	45.0	42.9	58.0	55.9	1491-1565 bp	1.0	58.3
trnS-GCU-trnG-UCC_p3	AGAAGAAGCGGAGAGAGAGG	AACTGAGAGACCCCTTAACT	55.0	45.0	58.2	55.1	1546-1620 bp	1.0	57.9
trnS-GCU-trnG-UCC_p4	CGGAGAGAGAGGGATTCGAA	AACTGAGAGACCCCTTAACT	55.0	45.0	58.3	55.1	1538-1612 bp	1.0	57.3
trnS-GCU-trnG-UCC_p5	AGAAGAAGCGGAGAGAGAGG	AAACTGAGAGACCCCTTAACT	55.0	42.9	58.2	55.9	1547-1621 bp	1.0	56.6