Hotspot

matK-trnK-UUU

LSC · 3295-4085 · 791 bp

Back to Borassus

Pi 0.00253

MarkerSeek score 60.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 2
pi: 0.00253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACGATCATGAGCAAATGGGT	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.6	58.2	918-920 bp	1.0	75.6
matK-trnK-UUU_p2	ACGATCATGAGCAAATGGGT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.6	58.2	917-919 bp	1.0	75.6
matK-trnK-UUU_p3	ACGATCATGAGCAAATGGGT	GTCGTGCAGTGCAATTCAAT	45.0	45.0	57.6	57.7	999-1001 bp	1.0	74.2
matK-trnK-UUU_p4	ACGATCATGAGCAAATGGGT	GGGTTGCTAACTCAACGGTA	45.0	50.0	57.6	57.5	931-933 bp	1.0	73.3
matK-trnK-UUU_p5	ACGATCATGAGCAAATGGGT	TTCGGGTTGCTAACTCAACG	45.0	50.0	57.6	58.5	934-936 bp	1.0	73.0