Hotspot

ndhF-rpl32

SSC · 115696-116663 · 968 bp

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Pi 0.00892

MarkerSeek score 64.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 968
variable_sites: 7
pi: 0.00892
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAATAAGCA	AGAAAAAGCTTTTATGGCAGC	45.0	38.1	57.8	55.6	984-1166 bp	1.0	56.3
ndhF-rpl32_p2	TGTTGCTGTCGGAATAAGCA	AAGAAAAAGCTTTTATGGCAGC	45.0	36.4	57.8	56.4	985-1167 bp	1.0	54.2
ndhF-rpl32_p3	TGTTGCTGTCGGAATAAGCA	TGGCAGCTAAATATCCCTTT	45.0	40.0	57.8	54.4	970-1152 bp	1.0	53.9
ndhF-rpl32_p4	TGTTGCTGTCGGAATAAGCA	TGGCAGCTAAATATCCCTTTT	45.0	38.1	57.8	55.2	970-1152 bp	1.0	52.9
ndhF-rpl32_p5	TGTTGCTGTCGGAATAAGCA	TGGCAGCTAAATATCCCTTTTTC	45.0	39.1	57.8	57.1	970-1152 bp	1.0	52.3