Hotspot

trnW-CCA-trnP-UGG

LSC · 69224-69391 · 168 bp

Back to Boophone

Pi 0.012

MarkerSeek score 56.40

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 2
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	234-235 bp	1.0	79.7
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	235-236 bp	1.0	79.7
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	223-224 bp	1.0	79.7
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	221-222 bp	1.0	79.7
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	224-225 bp	1.0	79.7