Hotspot

atpB-rbcL

LSC · 56624-57871 · 1248 bp

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Pi 0.0132

MarkerSeek score 53.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1248
variable_sites: 14
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	55.0	50.0	58.0	58.0	2033-2207 bp	1.0	84.5
atpB-rbcL_p2	GCCGACTGGAACACTTAGAG	AGATCCTCCAGACGTAGAGC	55.0	55.0	58.0	58.0	1796-1970 bp	1.0	84.5
atpB-rbcL_p3	GCCGACTGGAACACTTAGAG	ATCCTCCAGACGTAGAGCTC	55.0	55.0	58.0	58.0	1794-1968 bp	1.0	84.5
atpB-rbcL_p4	GCCGACTGGAACACTTAGAG	GATCCTCCAGACGTAGAGCT	55.0	55.0	58.0	58.0	1795-1969 bp	1.0	84.5
atpB-rbcL_p5	GCCGACTGGAACACTTAGAG	GTCCACACAGTTGTCCATGT	55.0	50.0	58.0	58.0	1595-1769 bp	1.0	84.5