Hotspot

trnS-GCU-trnG-GCC

LSC · 8348-9518 · 1171 bp

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Pi 0.0145

MarkerSeek score 62.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1171
variable_sites: 16
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGTCGCTTTAGTCCA	CGGACGAATCACACTTTTACC	50.0	47.6	58.0	57.3	1192-1243 bp	1.0	71.3
trnS-GCU-trnG-GCC_p2	AATCCGTCGCTTTAGTCCAC	CGGACGAATCACACTTTTACC	50.0	47.6	58.0	57.3	1191-1242 bp	1.0	71.3
trnS-GCU-trnG-GCC_p3	AGCAATCCGTCGCTTTAGTC	CGGACGAATCACACTTTTACC	50.0	47.6	58.4	57.3	1194-1245 bp	1.0	68.4
trnS-GCU-trnG-GCC_p4	CGTCGCTTTAGTCCACTCAG	CGGACGAATCACACTTTTACC	55.0	47.6	58.4	57.3	1187-1238 bp	1.0	68.3
trnS-GCU-trnG-GCC_p5	GCTTTAGTCCACTCAGCCAT	CGGACGAATCACACTTTTACC	50.0	47.6	57.6	57.3	1183-1234 bp	1.0	68.0