Hotspot

ycf1

SSC · 122431-128013 · 5583 bp

Back to Bonnaya

Pi 0.0166

MarkerSeek score 48.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5583
variable_sites: 138
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CATTCCTGACAGCCCTTTCA	AGGAACAAGAGGCATTCAGC	50.0	50.0	57.8	58.2	357-363 bp	1.0	81.9
ycf1_p2	CATTCCTGACAGCCCTTTCA	GGGAGAATACCCTCACCCAT	50.0	55.0	57.8	58.2	478-484 bp	1.0	81.1
ycf1_p3	TCATTCCTGACAGCCCTTTC	AGGAACAAGAGGCATTCAGC	50.0	50.0	57.5	58.2	358-364 bp	1.0	79.5
ycf1_p4	CATTCCTGACAGCCCTTTCA	TCGGAAGTGAAGGGGACTAA	50.0	50.0	57.8	57.7	385 bp	0.666667	63.6
ycf1_p5	CATTCCTGACAGCCCTTTCA	TTCGGAAGTGAAGGGGACTA	50.0	50.0	57.8	57.7	386 bp	0.666667	63.6