Hotspot

ndhF

SSC · 109113-111371 · 2259 bp

Back to Bonnaya

Pi 0.0115

MarkerSeek score 50.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 39
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AATACCCCTTTCGGATCGGA	CCAATACCCCCTCCTTTTCC	50.0	55.0	58.2	57.5	2920-2927 bp	1.0	76.4
ndhF_p2	ATACCCCTTTCGGATCGGAA	CCAATACCCCCTCCTTTTCC	50.0	55.0	58.2	57.5	2919-2926 bp	1.0	76.4
ndhF_p3	ATACCCCTTTCGGATCGGAA	CCCAATACCCCCTCCTTTTC	50.0	55.0	58.2	57.5	2920-2927 bp	1.0	76.4
ndhF_p4	TACCCCTTTCGGATCGGAAT	CCAATACCCCCTCCTTTTCC	50.0	55.0	58.2	57.5	2918-2925 bp	1.0	76.4
ndhF_p5	TACCCCTTTCGGATCGGAAT	CCCAATACCCCCTCCTTTTC	50.0	55.0	58.2	57.5	2919-2926 bp	1.0	76.4