Hotspot

accD

LSC · 58186-59727 · 1542 bp

Back to Bonnaya

Pi 0.0125

MarkerSeek score 54.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1542
variable_sites: 29
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAAATAAAGAATTGTGGATCGA	CGAAAAATTGAACTTAGTGCGC	27.3	40.9	52.1	57.5	1587 bp	1.0	41.5
accD_p2	AAAAATAAAGAATTGTGGATCGA	CGAAAAATTGAACTTAGTGCGC	26.1	40.9	53.0	57.5	1588 bp	1.0	41.5
accD_p3	TAAAAATAAAGAATTGTGGATCGA	CGAAAAATTGAACTTAGTGCGC	25.0	40.9	53.0	57.5	1589 bp	1.0	41.5
accD_p4	AAAATAAAGAATTGTGGATCGA	CGAAAAATTGAACTTAGTGCGCT	27.3	39.1	52.1	58.8	1587 bp	1.0	41.5
accD_p5	AAAATAAAGAATTGTGGATCGA	TCGAAAAATTGAACTTAGTGCGC	27.3	39.1	52.1	58.8	1588 bp	1.0	41.5