Hotspot

rps19-rpl2

IRb · 84583-84645 · 63 bp

Back to Bonnaya

Pi 0.0635

MarkerSeek score 84.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 63
variable_sites: 6
pi: 0.0635
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATTCACGAGACCAAG	CACAACCCCTTGGGGTTATC	47.6	55.0	57.1	58.2	275-280 bp	1.0	65.6
rps19-rpl2_p2	GGTAGATTCACGAGACCAAGT	CACAACCCCTTGGGGTTATC	47.6	55.0	57.1	58.2	274-279 bp	1.0	65.6
rps19-rpl2_p3	TGGTAGATTCACGAGACCAAG	TTGGGGTTATCCTGCACTTG	47.6	50.0	57.1	57.8	266-271 bp	1.0	65.1
rps19-rpl2_p4	GGTAGATTCACGAGACCAAGT	TTGGGGTTATCCTGCACTTG	47.6	50.0	57.1	57.8	265-270 bp	1.0	65.1
rps19-rpl2_p5	GATGGTAGATTCACGAGACCA	CACAACCCCTTGGGGTTATC	47.6	55.0	56.9	58.2	277-282 bp	1.0	63.9