Hotspot

psbB-psbT

LSC · 74485-74675 · 191 bp

Back to Bonnaya

Pi 0.0262

MarkerSeek score 65.20

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 7
pi: 0.0262
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCCAAAAACTTGGGGATCCA	GAACTTTAGGCGGTTCTCGA	45.0	50.0	56.9	57.6	322-327 bp	1.0	70.4
psbB-psbT_p2	TCCAAAAACTTGGGGATCCA	TGGAACTTTAGGCGGTTCTC	45.0	50.0	56.9	57.5	324-329 bp	1.0	69.6
psbB-psbT_p3	TTCCAAAAACTTGGGGATCCA	GAACTTTAGGCGGTTCTCGA	42.9	50.0	57.7	57.6	323-328 bp	1.0	68.4
psbB-psbT_p4	TCCAAAAACTTGGGGATCCA	GGAACTTTAGGCGGTTCTCG	45.0	55.0	56.9	58.6	323-328 bp	1.0	68.2
psbB-psbT_p5	TCCAAAAACTTGGGGATCCA	AGTTGGAACTTTAGGCGGTT	45.0	45.0	56.9	57.3	327-332 bp	1.0	68.1