Hotspot

atpH-atpI

LSC · 13881-14850 · 970 bp

Back to Bonnaya

Pi 0.0125

MarkerSeek score 67.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 970
variable_sites: 18
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1042-1056 bp	1.0	77.5
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1060-1074 bp	1.0	77.1
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1059-1073 bp	1.0	76.5
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.3	58.5	1054-1068 bp	1.0	76.2
atpH-atpI_p5	AACGGAAGCAGCAGAAATCA	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.1	57.3	1027-1041 bp	1.0	75.8