Hotspot

rps14

LSC · 38087-38398 · 312 bp

Back to Bonia

Pi 0
MarkerSeek score 19.80
Primer available yes
Label rps14

Feature Metadata

label_name
rps14
feature_type
gene
region
LSC
length_bp
312
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
19.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps14_p1 TAGCAAGACATTCACCGAGC GTATGGGCGTGGATGTTCTT 50.0 50.0 58.0 58.0 1358-1413 bp 1.0 80.0
rps14_p2 TAGCAAGACATTCACCGAGC TTTCCTGGCGTGGATATTGG 50.0 50.0 58.0 57.9 1297-1352 bp 1.0 79.9
rps14_p3 TAGCAAGACATTCACCGAGC TAGTTTTCCTTGTGACGGCC 50.0 50.0 58.0 58.1 1626-1681 bp 1.0 79.4
rps14_p4 AGCCTTGTGAGCTACCAAAC GTATGGGCGTGGATGTTCTT 50.0 50.0 58.1 58.0 880-936 bp 1.0 79.4
rps14_p5 AGCCTTGTGAGCTACCAAAC TTTCCTGGCGTGGATATTGG 50.0 50.0 58.1 57.9 819-875 bp 1.0 79.3