Hotspot

psbH

LSC · 72639-72860 · 222 bp

Back to Bonia

Pi 0.0045
MarkerSeek score 55.10
Primer available yes
Label psbH

Feature Metadata

label_name
psbH
feature_type
gene
region
LSC
length_bp
222
variable_sites
1
pi
0.0045
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH_p1 CGTGTATTTACAACTACAACGGA AATTCCACGGTCGAACTACC 39.1 50.0 56.9 57.6 389 bp 1.0 44.6
psbH_p2 TCGTGTATTTACAACTACAACGG AATTCCACGGTCGAACTACC 39.1 50.0 56.9 57.6 390 bp 1.0 44.6
psbH_p3 TCGTGTATTTACAACTACAACGGA AATTCCACGGTCGAACTACC 37.5 50.0 58.2 57.6 390 bp 1.0 43.3
psbH_p4 CGTGTATTTACAACTACAACGGA ACGGTCGAACTACCAGAATG 39.1 50.0 56.9 57.4 383 bp 1.0 42.4
psbH_p5 CGTGTATTTACAACTACAACGGA CGGTCGAACTACCAGAATGT 39.1 50.0 56.9 57.4 382 bp 1.0 42.4