Hotspot

rpl32-trnL-UAG

SSC · 107016-107734 · 719 bp

Back to Bonia

Pi 0.00975
MarkerSeek score 56.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
719
variable_sites
7
pi
0.00975
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 802-803 bp 1.0 78.8
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 801-802 bp 1.0 78.8
rpl32-trnL-UAG_p3 AAGATCATTTTCCAGCGGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 839-840 bp 1.0 78.5
rpl32-trnL-UAG_p4 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 795-796 bp 1.0 78.3
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 794-795 bp 1.0 78.3