Hotspot

rpl20-rps12

LSC · 68442-69137 · 696 bp

Back to Bonia

Pi 0.00433
MarkerSeek score 61.70
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
696
variable_sites
3
pi
0.00433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACGCATTTTTGTTCGTCGTC GAGGAACATGTGCTAGGGTG 45.0 55.0 58.0 58.0 770-773 bp 1.0 80.3
rpl20-rps12_p2 ACGCATTTTTGTTCGTCGTC CGACATGCCAACGGTTAAAC 45.0 50.0 58.0 58.0 865-868 bp 1.0 80.1
rpl20-rps12_p3 ACGCATTTTTGTTCGTCGTC GTCGAGGAACATGTGCTAGG 45.0 55.0 58.0 58.1 773-776 bp 1.0 79.9
rpl20-rps12_p4 GTTTGAGGCAAATGAACGCA GAGGAACATGTGCTAGGGTG 45.0 55.0 57.9 58.0 785-788 bp 1.0 79.3
rpl20-rps12_p5 GTTTGAGGCAAATGAACGCA CGACATGCCAACGGTTAAAC 45.0 50.0 57.9 58.0 880-883 bp 1.0 79.1