Hotspot

rps14-psaB

LSC · 38399-38499 · 101 bp

Back to Bonia

Pi 0.0594
MarkerSeek score 82.40
Primer available yes
Label rps14-psaB

Feature Metadata

label_name
rps14-psaB
feature_type
igs
region
LSC
length_bp
101
variable_sites
6
pi
0.0594
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps14-psaB_p1 CTCAAGGGGGAAACTTTGCT GCAGCTTTCTTGATTGCCTC 50.0 50.0 58.0 57.7 259-315 bp 1.0 79.5
rps14-psaB_p2 CTCAAGGGGGAAACTTTGCT GCTCTTTCCATTGTGCAAGC 50.0 50.0 58.0 58.3 328-384 bp 1.0 79.0
rps14-psaB_p3 CTCAAGGGGGAAACTTTGCT TGGCTCTTTCCATTGTGCAA 50.0 45.0 58.0 58.3 330-386 bp 1.0 78.9
rps14-psaB_p4 CTCAAGGGGGAAACTTTGCT TGCAGCTTTCTTGATTGCCT 50.0 45.0 58.0 58.4 260-316 bp 1.0 78.7
rps14-psaB_p5 CTCAAGGGGGAAACTTTGCT GGCTCTTTCCATTGTGCAAG 50.0 50.0 58.0 57.6 329-385 bp 1.0 78.5