Hotspot

psbM-petN

LSC · 17949-18698 · 750 bp

Back to Bonia

Pi 0.00136
MarkerSeek score 61.30
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
750
variable_sites
1
pi
0.00136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGCTACTGCACTGTTCATTCT TAGTAAGTCTCGCTTGGGCT 42.9 50.0 57.6 58.2 898-912 bp 1.0 68.9
psbM-petN_p2 TGCTACTGCACTGTTCATTCT GTAGTATGGGGGAGGAGTGG 42.9 60.0 57.6 58.3 842-856 bp 1.0 67.9
psbM-petN_p3 TGCTACTGCACTGTTCATTCT TAGTATGGGGGAGGAGTGGA 42.9 55.0 57.6 58.4 841-855 bp 1.0 67.2
psbM-petN_p4 TGCTACTGCACTGTTCATTC TAGTAAGTCTCGCTTGGGCT 45.0 50.0 56.1 58.2 898-912 bp 1.0 64.7
psbM-petN_p5 TGCTACTGCACTGTTCATTCT AGTAGTATGGGGGAGGAGTG 42.9 55.0 57.6 57.2 843-857 bp 1.0 63.9