Hotspot

trnG-UCC-trnT-GGU

LSC · 12539-15533 · 2995 bp

Back to Bonia

Pi 0.00502
MarkerSeek score 56.80
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
2995
variable_sites
15
pi
0.00502
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 GAAAACCCATAGCCAGGAGG GGACGGATTTGTTGAAGCGA 55.0 50.0 57.9 58.8 242 bp 1.0 74.4
trnG-UCC-trnT-GGU_p2 CCCATAGCCAGGAGGAGTTA GGACGGATTTGTTGAAGCGA 55.0 50.0 57.9 58.8 237 bp 1.0 74.1
trnG-UCC-trnT-GGU_p3 TTTTTGTTGGGCAGGCAGTA GGACGGATTTGTTGAAGCGA 45.0 50.0 58.2 58.8 193 bp 1.0 73.6
trnG-UCC-trnT-GGU_p4 GAAAACCCATAGCCAGGAGG TTGGACGGATTTGTTGAAGC 55.0 45.0 57.9 56.9 244 bp 1.0 72.5
trnG-UCC-trnT-GGU_p5 AAAACCCATAGCCAGGAGGA GGACGGATTTGTTGAAGCGA 50.0 50.0 58.3 58.8 241 bp 1.0 72.3