Hotspot

rpl32-trnL-UAG

SSC · 119918-121136 · 1219 bp

Back to Bombycidendron

Pi 0.00921

MarkerSeek score 65.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1219
variable_sites: 11
pi: 0.00921
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1313-1334 bp	1.0	80.7
rpl32-trnL-UAG_p2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1314-1335 bp	1.0	80.7
rpl32-trnL-UAG_p3	CGGCGTTGAAAGCTTTTTCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	1323-1344 bp	1.0	79.8
rpl32-trnL-UAG_p4	AAGAAAGGATATTGGGCGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	1329-1350 bp	1.0	79.6
rpl32-trnL-UAG_p5	AAGAAAGGATATTGGGCGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	1330-1351 bp	1.0	79.6