Hotspot

ndhF-rpl32

SSC · 118569-119752 · 1184 bp

Back to Bombycidendron

Pi 0.00852

MarkerSeek score 64.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1184
variable_sites: 10
pi: 0.00852
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGAAGTGGCACAAAAGGTA	TGAAAAAGCTTTCAACGCCG	50.0	45.0	58.0	58.2	1331-1334 bp	1.0	79.8
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	TGAAAAAGCTTTCAACGCCG	45.0	45.0	58.0	58.2	1379-1382 bp	1.0	79.4
ndhF-rpl32_p3	GGGAAGTGGCACAAAAGGTA	GCCGCCCAATATCCTTTCTT	50.0	50.0	58.0	58.3	1315-1318 bp	1.0	78.7
ndhF-rpl32_p4	TGTTGCCGTCGGAAAAAGTA	GCCGCCCAATATCCTTTCTT	45.0	50.0	58.0	58.3	1363-1366 bp	1.0	78.3
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TGAAAAAGCTTTCAACGCCG	45.0	45.0	57.8	58.2	1381-1384 bp	1.0	78.3